Optical SENSING Applied to Decellularization
renal
Name: RAQUEL AHNERT AGUIAR EVANGELISTA
Publication date: 14/12/2020
Advisor:
Name |
Role |
|---|---|
| BRENO VALENTIM NOGUEIRA | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| BRENO VALENTIM NOGUEIRA | Advisor * |
| TEODIANO FREIRE BASTOS FILHO | Internal Examiner * |
Summary: The kidney is the most requested organ for transplants in Brazil. Currently, 68% of
patients on the transplant waiting list are waiting for this organ. Decellularization
technique has emerged as a promising alternative for increasing supply and
decreasing transplant rejection rates. The concentration of residual DNA is the main
parameter to certify the quality of the scaffold. However, it is an invasive and
destructive technique. To follow the process during decellularization without damaging,
Pereira et al (2019) developed an optical monitoring system composed of three
modules (light source, photodetector and prototyping plate) coupled to a computer with
own programming. This system records the organs optical profile in the form of voltage
graphs (v.s-1). Our objective was to analyze the graphic records produced by the
sensor after renal decellularization, and relate them to the final organ quality. We used
kidneys of adult wistar rats with average mass of 301.8 ± 44.6 g. Decellularizations
occurred within the optical sensor and native and decellularized organs were
separated for DNA analysis, hydroxyproline dosage and histology. The curves
produced by the sensor were smoothed to reduce noise and adjusted according to a
four-parameter logistic regression model (A1, A2, S and X0), described by Gadagkar
and Call (2000). DNA analysis confirmed 95% of cell removal and the hydroxyproline
dosage confirmed the maintenance of tissue collagen. The optical profiles produced
an S curve pattern, and the parameter X0 was the one that obtained the best
correlation with the residual DNA (52%).
