EVALUATION OF CHLOROPHYLL FLUORESCENCE AS A STRESS INDICATOR TOOL IN Schinus terebinthifolia Raddi.
Name: RHAIRA RODRIGUES DALLA
Publication date: 31/03/2025
Examining board:
Name |
Role |
|---|---|
| FLAVIA IMBROISI VALLE ERRERA | Examinador Interno |
| MARCO CESAR CUNEGUNDES GUIMARAES | Presidente |
| MARCOS BARROS MEDEIROS | Examinador Externo |
Summary: The aim of this study was to evaluate the effectiveness of chlorophyll fluorescence as a diagnostic tool to identify and monitor abiotic stress in Schinus terebinthifolia Raddi. In addition, we sought to correlate changes in fluorescence indices and the modulation of proteins involved in the protection and adaptation of the photosynthetic apparatus. In vivo analyses of chlorophyll fluorescence were performed using a portable fluorometer at four stations located in Espírito Santo (AE1, AE2, AE3 and AE4) in plants under abiotic stress conditions. This allowed obtaining parameters such as maximum photosynthetic efficiency (Fv/Fm) and non-photosynthetic energy dissipation (NPQ), evaluated by OJIIP and SPAD. In addition, a search was carried out at NCBI for potential proteins to be used in the development of diagnostic tests in plants under mineral stress. The results of the study demonstrated that the exposure of Schinus terebinthifolia Raddi to different abiotic stress conditions caused marked changes in
photosynthetic efficiency, and a significant reduction in the maximum photosynthetic efficiency indexes (Fv/Fm) was also observed, indicating damage to the photosynthetic system resulting from the applied stresses. In addition, five proteins, OHP1, OHP2, PSBS, ELIP1 and cpSRP43, were identified as potential proteins to be tested in a greenhouse using Arabidopsis thaliana as a model organism. Thus, the study showed that chlorophyll fluorescence is a promising tool for the diagnosis of abiotic stress in Schinus terebinthifolia Raddi, allowing the early detection of imbalances in the photosynthetic apparatus. Furthermore, the mentioned proteins can be applied in future studies because they are associated with the construction of
the photosynthetic apparatus and interaction with pigments for chlorophyll synthesis.
