Evaluation of the cytotoxicity effect of microplastics on the human mammary cell line MCF10A
Name: LAÍZA DOS SANTOS RIBEIRO DA SILVA
Publication date: 10/03/2025
Examining board:
Name |
Role |
|---|---|
| ELDAMARIA DE VARGAS WOLFGRAMM DOS SANTOS | Presidente |
| FLAVIA DE PAULA | Examinador Interno |
| MARCELLA PORTO TAVARES | Examinador Externo |
Summary: Introduction: It is known that the use of plastic and its derivatives has been growing exponentially, accompanied by advances involving poor management, detachment and degradation of plastic waste. Studies also indicate the presence of components
such as microplastic in the human body, which may pose health risks. Additionally, antioxidant compounds, such as resveratrol, have been investigated as potential attenuator of toxic effects. Given this, investigating the impacts of microplastics and
compounds such as resveratrol is essential to understanding the implications for human health. Objective: To evaluate the impact of microplastics on MCF10A cells and the attenuating potential of resveratrol. Methodology: This is a laboratory study using MCF10A cell culture exposed to six different concentrations of MPs (20 m and 1 m), RSV exposure at six different concentrations, and a mixed group (combining 20 m MPs and RSV) at different concentrations. Cell viability was analyzed at 24h and 72h time points through a specific assay. Results: The isolated exposure of cells to MPs (20 and 1 m) did not show significant changes in cell viability at any time point. However, an increase in cell proliferation was observed when exposed to 1 m MPs. Regarding RSV, cell viability was reduced to 11.74% after 72h of exposure at a concentration of 500 M. In the mixed treatment, cell viability was reduced to 46.90% (p<0.0025) with 500 g/mL MPs + 250 M RSV and to 11.36% (p<0.0001) with 1000 g/mL MPs + 500 M RSV, suggesting a potential interaction between the compounds. Furthermore, the IC50 of RSV was identified as 263.8 M at 72h. Conclusion: The results showed that MPs (20 and 1 M) did not have significant cytotoxic effects at
both times; while RSV, at high concentrations, reduced cell viability and presented an IC50 of 263.8 M. The combination of MPs and RSV indicates a possible synergistic interaction, increasing cytotoxicity at high concentrations. Further studies are needed
to better understand the impact of MPs on human health and the mechanisms involved.
