THERAPEUTIC POTENTIAL OF L-ARGININE IN THE OXIDATIVE SYSTEM IN MDA-MB-231 TRIPLE NEGATIVE BREAST CANCER CELLSABSTRACT
Name: JOÃO AUGUSTO DINIZ MOURA
Publication date: 18/03/2025
Examining board:
Name |
Role |
|---|---|
| CARMEM LUISA SARTÓRIO | Examinador Externo |
| DEBORA DUMMER MEIRA | Examinador Interno |
| SONIA ALVES GOUVEA | Presidente |
Summary: Antineoplastic therapeutic strategies are the focus of numerous studies due to the complexity of treatments, which, although often effective, can exacerbate comorbidities through adverse effects that impact patient prognosis and survival. In vitro studies with tumor cells and in vivo studies using L-arginine in cancer patients have shown beneficial results due to its effects on multiple physiological systems. However, it is crucial to investigate the specific pathways that directly contribute to reducing tumor
progression. In this context, the MDA-MB-231 triple-negative breast cancer cell line— characterized by high aggressiveness and limited treatment options due to the absence of a therapeutic target—serves as a valuable model for evaluating the effects
of different L-arginine (L-ARG) concentrations on cell viability and the oxidative system. Cells were treated with L-ARG at concentrations of 800 g/mL, 1600 g/mL, and 3200 g/mL for 48 hours. The dihydroethidium (DHE) staining assay was performed to detect the increased presence of reactive oxygen species (ROS), specifically the superoxide anion (O2). The AlamarBlue assay was used to measure cell proliferation and viability. Data analysis was conducted using GraphPad Prism® 8.0.2 software.
