Development of a decellularized bone biomaterial enriched with fetal bovine bone hydrogel
Name: RODOLPHO JOSÉ DA SILVA BARROS
Publication date: 10/10/2024
Examining board:
Name |
Role |
|---|---|
| BRENO VALENTIM NOGUEIRA | Presidente |
| CARLOS MAGNO DA COSTA MARANDUBA | Examinador Externo |
| JAIRO PINTO DE OLIVEIRA | Examinador Externo |
| MARCO CESAR CUNEGUNDES GUIMARAES | Examinador Interno |
| RAFAELA AIRES | Examinador Externo |
Summary: The global bone graft material market is projected to grow at a rate of 6-7% annually until 2032. Conventional bone grafting strategies are no longer considered sufficient or adequate for most treatments. The development of decellularized biomaterials
derived from animal tissues (xenografts) offers a promising and viable strategy. The structural similarity between animal and human bones, along with the decellularization technique, can result in a high-quality biomaterial that provides mechanical support, preserves the organic matrix, and supplies essential biological stimuli for tissue regeneration. Adult and fetal bovine bones were decellularized to produce biomaterials (blocks, granules, and hydrogel) with residual DNA levels lower than 50 ng/mg and SDS levels below 2 g/mg of dry tissue. Thermogravimetric assays (DSC and TGA) and infrared spectroscopy (FTIR and Raman) demonstrated the adequate preservation of both the mineral and organic bone matrices. Scanning electron microscopy (SEM) and elemental composition analysis (EDS and CHNS-O) confirmed the preservation of the microstructure and biochemical composition of the bone matrix. pH stability, absence of heavy metals, and sterility were verified. Comparative tests showed that the decellularized biomaterials, particularly those derived from fetal bone, exhibit superior porosity, density, mechanical support
capacity, and organic composition compared to other biomaterials. In vitro tests revealed no cytotoxicity and a higher ability to stimulate the proliferation of mesenchymal cells and fibroblasts. The results indicate that the production of decellularized bovine bone biomaterials coated with fetal hydrogel could contribute to better biocompatibility of implants and help meet the global demand for bone graft materials.
