EVALUATION OF BONE BIOMATERIAL CYTOTOXICITY DECELLULARIZED
Name: AFFONSO MARTINHO BURINI BORTOLOTTI FAVALESSA
Publication date: 14/04/2023
Advisor:
Name |
Role |
|---|---|
| BRENO VALENTIM NOGUEIRA | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| BRENO VALENTIM NOGUEIRA | Advisor * |
| FLAVIA DE PAULA (M/D) | Internal Examiner * |
Summary: The characteristic of bone defects of different origins is still a challenge in the medical and dental fields. Currently, several bone substitutes are used in exertion procedures resulting from injury or trauma. However, only a few manage to promote biochemistry with the organism, being the targets of bone tissue research. In view of this, tissue bioengineering is responsible for evaluating the capacity for osseointegration, direct interaction between the bone and the improved biomaterial, which promotes rapid adherence and greater quality in the procedure. Previous research results from the Núcleo de Biotecnologia Tcidual suggest producing a decellularized scaffold from fetal bone tissue with ideal morphological characteristics to be a new and promising bone biomaterial. Continuing the research, this project aims to evaluate the interaction of the scaffold when in contact with mesenchymal stem cells (MSC). Through in vitro assays, this work investigated the biocompatibility of the biomaterial to add knowledge in the clinical testing phase. The decellularization of the bovine fetal bone biomaterial was performed and then the residual DNA and SDS value, elemental composition and cytotoxicity test with human cells derived from the stromal mesenchyme of adipose tissue were analyzed. Finally, this work successfully evaluated the manufacture of the biomaterial and compared it with a similar product already on the market. Biocompatibility was approved, however residual SDS values may have hindered its performance.
