Name: JULIA DEL PIERO PEREIRA
Publication date: 23/02/2023
Advisor:
Name |
Role |
|---|---|
| ELDAMÁRIA DE VARGAS WOLFGRAMM DOS SANTOS | Co-advisor * |
| IURI DRUMOND LOURO (M/D) | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| FLAVIA DE PAULA (M/D) | Internal Examiner * |
| IURI DRUMOND LOURO (M/D) | Advisor * |
Summary: The present study was conducted with the participation of 4 volunteers who made themselves available to produce fingerprints on previously sanitized plastic and wood surfaces, in order to leave contact DNA. The traces left in the matrices were subjected to different conditioning variables such as the incidence of direct and indirect sunlight, as well as the time between contact and collection of 24h and 72h. The collection was performed using a double swab and its DNA was extracted through organic extraction with phenol-chloroform. Then the material was quantified by nanodrop, and the samples with the best results passed through another spectrophotometer, the qubit. In
addition to contact DNA samples, the entire process was carried out with negative control samples, that is, samples from surfaces cleaned with 70% alcohol and 20 minutes of UV light incidence prior to fingerprinting. As well as a positive control, that is, oral swab samples from each study subject. Data were tabulated and submitted to statistical tests.
