DETECTION OF POTENTIAL HOST PLANTS OF THE PAPAYA
STICKY DISEASE VIRUSES COMPLEX (PMeV E PMeV-2)

Name: Marcos Vinicius Hell
Type: MSc dissertation
Publication date: 31/03/2021
Advisor:

Namesort descending Role
Jose Aires Ventura (M/D) Advisor *

Examining board:

Namesort descending Role
Diolina Moura Silva Internal Examiner *
Helcio Costa External Examiner *
Jose Aires Ventura (M/D) Advisor *
Patricia Machado Bueno Fernandes (M/D) Co advisor *

Summary: HELL, M. V. DETECTION OF POTENTIAL HOST PLANTS OF THE PAPAYA
STICKY DISEASE VIRUSES COMPLEX (PMeV E PMeV-2) 2020. 36f. Dissertation
(Master in Biotechnology) - Graduate Program in Biotechnology, UFES, Espírito
Santo. Brazil.
ABSTRACT
The papaya culture (Carica papaya L.) has an expressive participation in the national
fruit production, being the third most consumed fruit in the country. One of the main
diseases that affects papaya plantations in Brazil is the papaya sticky disease,
caused by the viral complex papaya meleira virus (PMeV) and papaya meleira virus 2
(PMeV2). Until then, the best strategy available to control the disease is to eradicate
infected plants. However, it is believed that the virus is able to remain in secondary
species, such as invasive and intercropped plants. The identification of model host
plants of the PMeV complex aims to provide advances in the study of virus-plant
interaction. Thus, the objective of this work was to identify host plants of the viral
complex. Eight species of plants (Citrullus lanatus, Cucurbita moschata, Nicotiana
benthamiana, Nicotiana tabacum, Phaseolus vulgaris, Vigna unguiculata, Momordica
charantia and Carica papaya) were inoculated, mechanically, by abrasive action and
injection in the stem apex, with inoculum obtained from latex of infected papaya
plants, with four replications. Additionally, in C. papaya, the inoculation was also
performed with the leaf cut. Samples were collected before inoculation and at 15, 30
and 45 days after inoculation for indexing by RT-PCR. At 15, 30 and 45 days after
inoculation, a visual assessment of the plants was performed, for local and systemic
symptoms. The species C. lanatus, C. moschata and N. benthamiana were positive
for the presence of PMeV and PMeV2 in the RT-PCR reaction. At 30 days, the plants
of C. lanatus and C. moschata showed lesions in the leaf margin and N.
benthamiana exhibited symptoms of chlorosis between the veins. There was no
difference between the inoculation methods in the plants of C. papaya. This is the
first time that host plants for the PMeV and PMeV2 viruses have been confirmed,
which enables new possibilities for investigating the plant virus interaction under
controlled conditions and establishing new disease management strategies.
Keywords: Carica papaya; Virus; Papaya sticky disease. Alternative hosts

Access to document

Acesso à informação
Transparência Pública

© 2013 Universidade Federal do Espírito Santo. Todos os direitos reservados.
Av. Marechal Campos, 1468 - Bonfim, Vitória - ES | CEP 29047-105