GENOMIC AND TRANSCRIPTOMIC ANALYSIS OF Saccharomyces cerevisiae UNDER CONSECUTIVE STRESSES

Name: Ane Catarine Tosi Costa
Type: PhD thesis
Publication date: 12/08/2021
Advisor:

Namesort descending Role
Patricia Machado Bueno Fernandes (M/D) Advisor *

Examining board:

Namesort descending Role
Alexandre Martins Costa Santos Internal Examiner *
Fernando Araripe Gonçalves Torres External Examiner *
Jose Aires Ventura (M/D) Internal Examiner *
Patricia Machado Bueno Fernandes (M/D) Advisor *
ROGELIO LOPES BRANDAO External Examiner *

Summary: GENOMIC AND TRANSCRIPTOMIC ANALYSIS OF Saccharomyces cerevisiae UNDER CONSECUTIVE STRESSES
ABSTRACT
COSTA, A.C.T. Genomic and transcriptomic analysis of of Saccharomyces cerevisiae under consecutive stresses. 2021. 123f. Thesis (Doctoral in Biotechnology) - Postgraduation Biotechnological Programme, UFES, Espírito Santo. Brazil.
During fermentation, yeasts face simultaneous and consecutive stresses, and their efficiency and performance depend on their adaptation to these unstable conditions. Even with the advent of omics analyzes, which allowed the complete yeast genome and its transcriptome to be studied, the response to consecutive stresses is still not well understood. To explore this, the genome and transcriptome of an industrial, stress-resistant strain (BT0510) was analyzed after consecutive stress and compared with other yeasts. Analysis of the genome of BT0510 identified several changes in genes involved in nitrogen metabolism and organelle integrity, as well as fragmentation of several flocculation genes. The strain also showed deletion of several genes encoding asparaginases, DUP240 family genes and maltose utilization loci. The common transcriptional response of BT0510 to different consecutive stresses indicated alterations in carbon metabolism, peroxisome activity, and oxidative stress response. Several genes were identified as key genes in the response, such as SYM1, STF2, and several HSPs along with the transcription factors ADR1 and USV1. Comparison of the transcriptome of BT0510 with a laboratory strain in response to the same stresses reinforced the role of ADR1 as well as SYM1 and several small HSPs. Comparative analysis of the transcriptome also suggested STF2 as a possible tolerance inducer in the industrial strain. Taken together, these results indicate target genes for the construction of more resistant strains that can optimize various fermentation processes.
Keywords: RNA-Seq. Yeast. Stress tolerance. Fermentative process. Genome. Transcriptome

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