Phenotypic and Molecular ASPECTS of Staphylococcus
aureus Isolated from Maxillofacial Bone Infection

Name: DALLIANE OLIVEIRA SOARES

Publication date: 21/12/2020
Advisor:

Name	Role
SANDRA LÚCIA VENTORIN VON ZEIDLER	Advisor *

Examining board:

Name	Role
ANA PAULA FERREIRA NUNES	Co advisor *
DANIEL CLAUDIO DE OLIVEIRA GOMES	Internal Examiner *
SANDRA LÚCIA VENTORIN VON ZEIDLER	Advisor *

Summary: Osteomyelitis is the main infectious disease that affects bone tissue. The most
frequent etiologic agent in this type of infection is Staphylococcus aureus, which can
be found in the normal microbiota of the skin and mucous membranes in humans,
but in favorable conditions, it becomes pathogenic establishing infection.
Osteomyelitis in the maxillofacial region, although not so common, has high
morbidity, low cure rates and often the need for multiple surgeries that can lead to
deformations and chronic sequelae, compromising the quality of life of patients, in
addition to presenting high hospital cost for adequate treatment and management.
Treatment for maxillofacial osteomyelitis is complex due to the high pathogenicity of
the strain through various mechanisms of action and the anatomy of the affected
bone itself. The aim of this work was to characterize 12 strains of S. aureus
phenotypically and genotypically, 6 isolated from maxillofacial osteomyelitis and 6
from asymptomatic nasal carriers. Additionally, the presence of the adhesin genes
Cna and Bbp, and the SCV phenotype were investigated in these strains. All strains
were genotypically confirmed by nuc gene expression. In this study, the adhesin
genes, Cna and Bbp, were not exclusive for the establishment of the infection and
the suggestive phenotype of SCVs was found in five of the six strains with infection
analyzed. We conclude that this subject is current and relevant for medical and
dental clinic. Our results are important for new studies to be carried out, testing new
adhesins and new methods for identifying the SCVs phenotype in S. aureus.

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