Golden Nanoparticles Reduced With Virola oleifera Inhibit the Growth of Staphylococcus aureus and Pseudomonas Aeruginosa
Name: RAFAELLA DE RESENDE MARQUES
Publication date: 18/11/2020
Advisor:
Name |
Role |
|---|---|
| MARCO CESAR CUNEGUNDES GUIMARÃES | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| ADRIANA MADEIRA ALVARES DA SILVA | Internal Examiner * |
| MARCO CESAR CUNEGUNDES GUIMARÃES | Advisor * |
Summary: The presente work analyzed activity antibacterial of gold nanoparticles reduced
with Virola oleífera (AuNPs-Vo). Cells of Staphylococcus aureus and
Pseudomonas aeruginosa (clinic and ATCC) are used for experiments. After
synthesis, AuNPs-Vo were characterizeds in localized surface plasmon
ressonance (LSPR), stability (ZETA), height and disperse (DLS e TEM). In
them, stability, floculation, ressuspention, antioxidant activities are avaliable.
AuNPs-Vo were ressuspended in differents mediuns (DMSO 5% e 10%, PBS, e
MH+) and solutions variable pH (1, 3, 5, 7, 9, 11 e 13) and concentration of
NaCl (1, 2,5, 5, 10, 25, 50, 100 e 200 mmol.mL-1
). Effects of differents
concentrations (900 450 225 112.5 62.5 µg/mL) of AuNPs-Vo in cells
developeneds (HaCaT e L929) and bacterials S. aureus e P. aeruginosa were
avaliable. Recents sinthesys of AuNPs-Vo showed height monodisperse 22 nm,
stables in differents mediuns (21,59 mv) and showed optimal condition for
insulation at 14000 rpm and 20 min.
AuNPs-Vo also showed high antioxidant activity and low toxicity. Finally, they
were effective in controlling the growth of both S. aureus and P. aeruginosas,
with values.
