Name: Juliana Dalbó
Type: PhD thesis
Publication date: 23/04/2020

Namesort descending Role
Adriana Madeira Alvares da Silva Advisor *

Examining board:

Namesort descending Role
Adriana Madeira Alvares da Silva Advisor *
Anderson Nogueira Mendes External Examiner *
Fábio Daumas Nunes External Examiner *
Greiciane Gaburro Paneto External Examiner *
Marco Cesar Cunegundes Guimarães Internal Examiner *

Summary: Action in combat oxidative stress and increase apoptosis in colorectal cancer cells
have been shown by the ingestion of bioactive compounds that constitute food and
juçara fruit. having seen that colorectal cancer is among the three main neoplasms
with the highest incidence and mortality rate worldwide. The present work aimed to
analyze the in vitro and in vivo effect of pulp juice and extract of phenolic substances
from juçara fruit in colorectal cancer. Ht-29 and Caco-2 cells were exposed to different
concentrations of pulp and extract of juçara fruit for 24 hours and analyzed by qRTPCR. For in vivo analysis, 43 male Wistar rats were used, divided in control group
(n=11) and induced to colorectal cancer (n=32). Juçara pulp juice and extract were
used for supplementation of rats induced by colorectal carcinogenesis. Body weight
gain, food consumption, biochemical and hematological profile were evaluated. Still,
foci of aberrant crypts and neoplastic lesions, mucins in colorectal mucosa,
immunohistochemistry and histopathological characterization of tubular
adenocarcinoma were analyzed. A significant decrease in gene expression of Nrf2,
Keap1, Bax and Caspase 3 was observed in the in vitro study in Ht-29 and Caco-2
cells exposed to extract and pulp of juçara fruit. In animals induced to carcinogenesis,
loss of body weight and lower food consumption were observed. There was also a
decrease in aspartate aminotransferase and serum albumin levels and increase in
alanine aminotransferase levels in the group supplemented with pulp. Low density
lipoproteins in the extract supplemented group also increased. In addition, there was
a 50.06% decrease in outbreaks of aberrant crypts attributed to supplementation with
pulp juice of juçara fruit. There was an increase in number of peyer plates in the group
supplemented with extract; percentage of acidic mucins and expression of Keap1 in
the group supplemented with pulp juice. By analyzing the results it was possible to
infer that juçara fruit increases the survival of cancer cells in vitro. In addition, it
significantly affects food consumption and body weight gain, with negative effects on
serum albumin and liver biochemical markers such as alanine aminotransferase and
low density lipoprotein. Therefore, juçara fruit pulp juice has a protective action in the
early stage of colorectal cancer development, acting to prevent the appearance of
aberrant crypts and to regulate the levels of aspartate aminotrasferase and Keap1, as
well as to protect the colic mucosa.

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