ALTA PRESSÃO HIDROSTÁTICA COMO FERRAMENTA DE SELEÇÃO DIRECIONADA EM S. cerevisiae PARA PRODUÇÃO DE ETANOL 2G PELA CASCA DE COCO VERDE

Name: LUÍZA FAVARATTO SANTOS

Publication date: 11/03/2019
Advisor:

Name	Role
ANTONIO ALBERTO RIBEIRO FERNANDES	Co-advisor *
PATRICIA MACHADO BUENO FERNANDES (M/D)	Advisor *

Examining board:

Name	Role
ALEXANDRE MARTINS COSTA SANTOS	Internal Examiner *
ANTONIO ALBERTO RIBEIRO FERNANDES	Co advisor *
PATRICIA MACHADO BUENO FERNANDES (M/D)	Advisor *

Summary: The green coconut shell is a pollutant residue of the environment generated to
tons in Brazil and that brings several environmental problems in its disposal.
Therefore, using it for the production of renewable energy is an important
sustainable solution for the mitigation of the environmental impact and for the
generation of energy. Yeast Saccharomyces cerevisiae represents one of the
microorganisms most used in the fermentation industries for the production of
biofuels from lignocellulolytic residues. There are already on the market strains
of this yeast capable of degrading lignocellulolytic residues, which have been
developed to increase the yield of the process, making it feasible financially on
an industrial scale. The high pressure hydrostatic (HHP) is a tool that simulates
three types of different stresses in the yeast: the ethyl, the thermal and the
oxidative, which are very common in fermentation dornas. In addition, studies
have shown that fermentation occurs more rapidly when the yeast is under
HHP. Therefore, HHP can be used as a targeted selection tool to obtain a strain
with higher fermentation capacity and greater resistance to fermentation
stresses, increasing the yield of the process. The aim of this work was to apply
a selective pressure to the S. cerevisiae strain G2-104 using high hydrostatic
pressure in order to obtain a strain with a higher fermentation capacity in green
coconut shell culture medium, to increase the yield of the second generation
ethanol production process.

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