DEVELOPMENT of Bone Biomaterial


Publication date: 26/02/2018

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Examining board:

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FLAVIA DE PAULA (M/D) Internal Examiner *

Summary: Despite the constant advancement in the development of alternatives for the
production of artificial experimental bone substitutes, the development of new
therapies that overcome the clinical efficacy of conventional bone grafts is still
considered a challenge for tissue bioengineering. The lack of implant biomaterials
capable of promoting mechanical and biological support of proven clinical efficacy
favors the use of deproteinized bone grafts that can not stimulate tissue
regeneration. In this context, the use of decellularized extracellular matrix (ECM) has
been considered a promising strategy for the development of tissue regeneration
therapies. Fetal bone tissue presents structural morphological features and
extracellular matrix composition that are very similar to growth tissues and bone
healing, so it can be explored as a promising candidate for the development of bone
subtitute biomaterials able to promote higher regenerative quality as implant devices.
To date, there have been no reports of the characterization and use of this type of
tissue, nor have the processing protocols described. The objective was to develop a
decellularized bone biomaterial produced from fetal bovine bone that has a
morphological composition and structure preserved for use in tissue bioengineering.
For this, a series of decellularization protocols were evaluated and standardized
using optical and electron microscopy techniques, as well as biochemical tests for
quantification of DNA, hydroxyproline, chondroitin sulfate, residual SDS, histological
and immunohistochemical analysis. The results presented in this study demonstrated
the effective decellularization of bovine fetal bone tissue treated with 0.5% SDS, with
a 96.2% reduction in DNA content, and preservation of extracellular matrix
components such as collagen, glycosaminoglycans and glycoproteins. In addition,
the decellularized scaffold of fetal bone tissue produced has been shown to have
morphological characteristics that are a possible and promising bone substitute
biomaterial that may be used for tissue bioengineering purposes.

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