ANALYSIS OF THE POTENTIAL OF OSTEOPONTIN AND SERPINA1 AS BIOMARKERS OF CARCINOMA EPIDERMÓIDE OF HEAD AND NECK
Name: CELENY MILAGROS FIGUERA GÓMEZ
Publication date: 22/02/2017
Advisor:
Name |
Role |
|---|---|
| SONIA ALVES GOUVEA | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| CAMILLE DE MOURA BALARINI | External Examiner * |
| FLAVIA DE PAULA (M/D) | Internal Examiner * |
| SONIA ALVES GOUVEA | Advisor * |
| SUELY GOMES DE FIGUEIREDO | External Examiner * |
Summary: Head and neck squamous cell carcinoma (HNSCC) is the most frequent histological
type of head and neck cancers. Despite the numerous studies, a common set of
biomarkers for HNSCC has not been established yet, evidencing the necessity to find
molecules that facilitate the prognosis and early diagnosis of this disease. The
objective of this research was to evaluate the Serpine1 and Osteopontin (OPN) as
biomarkers in HNSCC because they are poorly evaluated molecules in this cancer.
Biological samples and clinical-pathological data were collected from 40 patients with
HNSCC and 11 healthy individuals. The detection of Serpina1 and OPN mRNA
expression was performed by qPCR trials on tumor tissues and normal tissue
samples. The evaluation of Serpina1 and OPN protein expression was performed
with serum from both HNSCC patients and a control group by Western blotting. A
significant increase in Serpina1 mRNA was observed in tumor tissue compared to
normal tissue (p=0.0014) and in relation to different clinical-pathological
characteristics evaluated (sex, smoking and smoking, staging and lymph node
development). Serpina1 protein expression was also elevated in patients with
HNSCC in relation to controls (p=0.0007) and in clinical-pathological characteristics
evaluated. For OPN, no relevant significant differences were found for mRNA and
protein except OPN protein expression, which was shown higher in the advanced
stage (III/IV) (p=0.0181). Serpina1 has been shown as a potential biomarker of
HNSCC for alterations found in tumor tissue and blood, which may facilitate its
application in the development of the disease. The OPN should be better evaluated
to establish the possible significant differences.
Key words: Head and neck squamous cell carcinoma. Biomarkers. Osteopontin.
Serpina1.
