APPLICATION of the Hrm Technique on Identification of Fly Species (Diptera, Calliphoridae) of Forensic Importance
Name: PABLO VIANA OLIVEIRA
Publication date: 23/02/2018
Advisor:
Name |
Role |
|---|---|
| GREICIANE GABURRO PANETO | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| GREICIANE GABURRO PANETO | Advisor * |
| MARCO CESAR CUNEGUNDES GUIMARÃES | Internal Examiner * |
Summary: Forensic entomology consists of the study of insects and other arthropods in the solution of procedures related to criminal area. Most of entomological evidences are strongly dependent on accurate species identification. Due to problems based on morphological identification, new methods were developed, including molecular methods such as DNA Barcode. The objective of this work was to identify six species of forensic blow flies in Brazil. For this, the High Resolution Melting (HRM) technique was used. 44 samples of the species Chrysomya albiceps, Chrysomya megacephala, Chrysomya putoria, Cochliomyia macellaria, Lucilia cuprina and Lucilia eximia were sequenced by the cytochrome c oxidase subunit I (COI) gene, of which 70% of the samples were identified. Three pairs of primers were designed for amplification of the COI gene fragments with sizes of 124, 82 and 123 bp in regions with polymorphisms required for analysis by HRM. The HRM amplification protocol allowed to differentiate the six species of blow flies using a combination of amplicons of 82 and 124 bp, being effective in the identification of species of Calliphoridae family of forensic interest to Brazil and proved to be an alternative tool to the sequencing of DNA.
