EVALUATION OF THE BIOACTIVE POTENTIAL OF PEPTIDES FRACTIONS OBTAINED FROM TRIGGERFISH (Balistes capriscus) COPRODUCTS
Name: MONIQUE LOPES RIBEIRO
Publication date: 30/04/2024
Examining board:
Name | Role |
---|---|
BARTOLOMEU WARLENE SILVA DE SOUZA | Examinador Externo |
MARIA GABRIELA BELLO KOBLITZ | Examinador Externo |
MARIA APARECIDA CICILINI | Examinador Externo |
JAIRO PINTO DE OLIVEIRA | Examinador Externo |
ALEXANDRE MARTINS COSTA SANTOS | Presidente |
Summary: Fish industry can generate a significant amount of waste which have economic potential for use in pharmaceutical and food industry. Antioxidant and ACE-inhibitory activities of peptides fractions from triggerfish (Balistes capriscus) processing coproducts were evaluated. Protein fractions were extracted from fish viscera and hydrolysate using papain (HP), bromelain (HB) and trypsin (HT) (3% p.p-1, 6h). Molecular mass distribution of soluble protein extract (SPE) and hydrolysate were determined by gel filtration chromatography. Samples were extracted and fractionated (>100 MWCO, 30–100, 10-30 and < 10 MWCO). Antioxidant activity of fractions were evaluated and fraction SPE4 (<10 MWCO) showed the highest value of Trolox Equivalent Antioxidant Capacity - TEAC (10,157.7 mol Trolox. g-1) and Ferric Reducing Antioxidant Power - FRAP (1,588.71 mol FeSO4. g-1). SPE and hydrolysates (<10MWCO) were fractionated by ion-exchange chromatography and were subject to antioxidant activity assay. F1 fraction showed the highest value for TEAC capacity (8,839.04 mol Trolox. g-1) and FRAP (1,749.94 mol FeSO4. g-1). ACE-inhibitory activity was evaluated for non-hydrolysate and hydrolysate fractions and IC50 was determined. Fractions F3, F5 and HP3 showed the lowest IC50 value (30.1, 42.7 e 37.7 µg, respectively) using specific inhibitor captopril as control. It was possible to identify 19 amino acid sequences that could contribute to biological activity of the peptide fractions. Triggerfish peptides showed higher antioxidant and ACE inhibitory activities than previous studies with other species and could be a good source of bioactive peptides.