DEVELOPMENT OF DECLELLULARIZED FRAMEWORK FOR
use in Splenic Tissue Bioengineering

Name: TADEU ÉRITON CALIMAN ZANARDO

Publication date: 27/03/2020
Advisor:

Namesort descending Role
BRENO VALENTIM NOGUEIRA Advisor *

Examining board:

Namesort descending Role
BRENO VALENTIM NOGUEIRA Advisor *
DANIEL CLAUDIO DE OLIVEIRA GOMES External Examiner *
MARCO CESAR CUNEGUNDES GUIMARÃES Internal Examiner *

Summary: The spleen has already been considered a non-essential organ for life. However, its
importance is increasingly clear, given the serious disorders caused by its absence or
dysfunction, such as increased susceptibility to infections, thromboembolism and
cancer. In addition, it is known that its non-functionality may lead to patients already
afflicted with serious diseases such as HIV, myeloma and leukemia to have a higher
risk of infection and death. Therefore, we used the technique of decellularization to
obtain a viable splenic scaffold for recellularization and subsequent transplantation to
the host. In our study, we demonstrated that the scaffold created after the
decellularization process shows great removal of the DNA content and residual SDS,
which are essential and necessary to avoid immunogenic responses and failures after
transplantation to the host. In addition, there were preservation of the major
components of matrissome, such as collagens, glycoproteins and proteoglycans. In
the same way, we observed maintenance of important structural components such as
white pulp, marginal zone and red pulp, in addition to small, medium and large caliber
blood vessels. The scaffold developed by us was subsequently recellularized with
stromal cells from the spleen of neonate rats, WHERE we verified the ability of adhesion,
proliferation, viability and survival of cells in contact with the scaffold. Therefore, the
splenic scaffold proves to be very useful for studies that intend to use it for the recovery
of splenic function or even as a support for studies that intend to study changes in the
splenic extracellular matrix caused by diseases

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